IIKAWA Hiroko(Graduate School of Systems Life Sciences) paper has been accepted for Development Growth & Differentiation.
Congratulations!
Authors
Hiroko Iikawa, Aika Nishina, Mizuki Morita, Yuji Atsuta, Yoshiki Hayashi, Daisuke Saito
Affiliation
Graduate School of Systems Life Sciences
Manuscript Title
Labeling and sorting of avian primordial germ cells utilizing Lycopersicon Esculentum lectin
Abstract
Avian species are essential resources for human society, with their preservation and utilization heavily dependent on primordial germ cells (PGCs). However, efficient methods for isolating live PGCs from embryos remain elusive in avian species beyond chickens, and even in chickens, existing techniques have shown limited efficiency. In this study, we present a rapid, simple, and cost-effective method for labeling and sorting circulating-stage PGCs across various avian species, including Carinatae and Ratitae, using Lycopersicon Esculentum (Tomato) lectin (LEL). Notably, this method demonstrates high sorting efficiency by identifying a wide range of PGC subtypes while preserving the proliferative and migratory potential of chicken PGCs. This approach is anticipated to significantly contribute to the conservation, research, and agricultural industries related to avian species globally.
Journal name
Development Growth & Differentiation
Relevant SDGs
SDGs 3 (Good health and well-being)
Comments
Hello everyone, I am Hiroko Iikawa from system life sciences.
Here, I am sharing my paper which was freshly published. Mizuki Morita, one of the course student, also contributed this paper as third author.
This paper is not very complicated, therefore I hope you will try to read at once. Feel free to contact me for any comments and questions!
Contact
iikawa.hiroko.945★s.kyushu-u.ac.jp (Please convert ★ to @ to send the email)