Anamika Datta’s (Graduate School of Systems Life Sciences) paper has been accepted for the Cells. Congratulations!
Authors
Anamika Datta, Arif Istiaq, Shigehiko Tamura and Kunimasa Ohta
Affiliation
Stem Cell Biology, Systems Life Sciences
Manuscript Title
Bacterial Ribosomes Induce Plasticity in Mouse Adult Fibroblasts
Abstract
The incorporation of bacterial ribosome has been reported to induce multipotency in somatic and cancer cells which leads to the conversion of cell lineages. Queried on its universality, we observed that bacterial ribosome incorporation into trypsinized mouse adult fibroblast cells (MAF) led to the formation of ribosome-induced cell clusters (RICs) that showed strong positive alkaline phosphatase staining. Under in vitro differentiation conditions, RICs-MAF were differentiated into adipocytes, osteoblasts, and chondrocytes. In addition, RICs-MAF were able to differentiate into neural cells. Furthermore, RICs-MAF expressed early senescence markers without cell death. Strikingly, no noticeable expression of renowned stemness markers like Oct4, Nanog, Sox2, etc. was observed here. Later RNA-sequencing data revealed the expression of rare pluripotency-associated markers, i.e., Dnmt3l, Sox5, Tbx3 and Cdc73 in RICs-MAF and the enrichment of endogenous ribosomal status. These observations suggested that RICs-MAF might have experienced a non-canonical multipotent state during lineage conversion. In sum, we report a unique approach of an exo-ribosome-mediated plastic state of MAF that is amenable to multi-lineage conversion.
Journal name
Cells (MDPI)
Comments
Our latest paper shows incorporating bacterial ribosomes into mouse cells triggers a surprising cell lineage conversion! These cells gain the ability to become multiple cell types, like fat, bone, cartilages and even nerve cells. This unique state is different from typical stem cells and offers exciting possibilities for cell regeneration therapies. For interested readers, please check the mentioned link below.
